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expression vectors containing full-length human myc-ddk-tagged lepr and bbs9  (OriGene)


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    OriGene expression vectors containing full-length human myc-ddk-tagged lepr and bbs9
    Association of <t>BBS9</t> variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.
    Expression Vectors Containing Full Length Human Myc Ddk Tagged Lepr And Bbs9, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+human+myc+ddk+tagged+lepr/expression+vectors+containing+full+length+human+myc+ddk+tagged+lepr+and+bbs9/pmc08048836-85-8-12
    Average 90 stars, based on 1 article reviews
    expression vectors containing full-length human myc-ddk-tagged lepr and bbs9 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians"

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    Journal: Obesity (Silver Spring, Md.)

    doi: 10.1002/oby.23115

    Association of BBS9 variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.
    Figure Legend Snippet: Association of BBS9 variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.

    Techniques Used: Variant Assay

    Rare variants with a CADD score > 20 in known BBS genes, in which the mean BMI of carriers meets the threshold for severe obesity (≥ 40 kg/m 2 )
    Figure Legend Snippet: Rare variants with a CADD score > 20 in known BBS genes, in which the mean BMI of carriers meets the threshold for severe obesity (≥ 40 kg/m 2 )

    Techniques Used: Variant Assay

    BBS9 Thr549Ile carrier pedigrees. The black symbols in the pedigrees are heterozygous carriers at Thr549Ile; the white symbols are homozygous at Thr549Thr; the gray symbols are genotype unknown. Maximum BMI (kg/m 2 ), age at maximum BMI, and serum leptin (ng/mL) measures are shown for each participant, if known.
    Figure Legend Snippet: BBS9 Thr549Ile carrier pedigrees. The black symbols in the pedigrees are heterozygous carriers at Thr549Ile; the white symbols are homozygous at Thr549Thr; the gray symbols are genotype unknown. Maximum BMI (kg/m 2 ), age at maximum BMI, and serum leptin (ng/mL) measures are shown for each participant, if known.

    Techniques Used:

    The effect of the BBS9 variants on STAT3 transcription in HEK293 cells by an in vitro luciferase activity assay. Each bar represents the mean ± SD of luciferase activity, normalized to the leptin‐treated (100 ng/mL) BBS9 WT from five independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.
    Figure Legend Snippet: The effect of the BBS9 variants on STAT3 transcription in HEK293 cells by an in vitro luciferase activity assay. Each bar represents the mean ± SD of luciferase activity, normalized to the leptin‐treated (100 ng/mL) BBS9 WT from five independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Techniques Used: In Vitro, Luciferase, Activity Assay

    The effect of the BBS9 variants on STAT3 phosphorylation in HEK293 cells by Western blot analyses. Each bar represents the mean ± SD of signal intensity normalized to the leptin‐treated BBS9 WT; derived from three independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.
    Figure Legend Snippet: The effect of the BBS9 variants on STAT3 phosphorylation in HEK293 cells by Western blot analyses. Each bar represents the mean ± SD of signal intensity normalized to the leptin‐treated BBS9 WT; derived from three independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Techniques Used: Phospho-proteomics, Western Blot, Derivative Assay, Activity Assay

    Related Articles

    Expressing:

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians
    Article Snippet: .. Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland). .. The QuikChange Lightning Site‐Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) was used to create five different BBS9 (NM_198428) constructs with the following amino acid substitutions: exon7:c.G625T:p.V209L, exon16:c.A1633G:p.S545G, exon16:c.C1646T:p.T549I, exon19:c.C1993T:p.L665F, and exon21:c.A2428G:p.K810E.



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    expression vectors containing full-length human myc-ddk-tagged lepr and bbs9  (OriGene)
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    OriGene expression vectors containing full-length human myc-ddk-tagged lepr and bbs9
    Association of <t>BBS9</t> variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.
    Expression Vectors Containing Full Length Human Myc Ddk Tagged Lepr And Bbs9, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+human+myc+ddk+tagged+lepr/expression+vectors+containing+full+length+human+myc+ddk+tagged+lepr+and+bbs9/pmc08048836-85-8-12
    Average 90 stars, based on 1 article reviews
    expression vectors containing full-length human myc-ddk-tagged lepr and bbs9 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    length human myc ddk tagged lepr  (OriGene)
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    OriGene length human myc ddk tagged lepr
    Association of <t>BBS9</t> variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.
    Length Human Myc Ddk Tagged Lepr, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+human+myc+ddk+tagged+lepr/Leprot+(BC010289)+Mouse+Untagged+Clone/pmc08048836-85-3-12
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    Image Search Results


    Association of BBS9 variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    doi: 10.1002/oby.23115

    Figure Lengend Snippet: Association of BBS9 variant rs59252892 (Thr549Ile) and BMI. Bars represent the mean ± SD for the 13 Thr/Ile heterozygotes and 6,838 Thr/Thr homozygotes. The beta (β) log e scale corresponds to a 25% increase in BMI per copy of the risk allele. P values were adjusted for age, sex, birth year, and the first five genetic principal components.

    Article Snippet: Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland).

    Techniques: Variant Assay

    Rare variants with a CADD score > 20 in known BBS genes, in which the mean BMI of carriers meets the threshold for severe obesity (≥ 40 kg/m 2 )

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    doi: 10.1002/oby.23115

    Figure Lengend Snippet: Rare variants with a CADD score > 20 in known BBS genes, in which the mean BMI of carriers meets the threshold for severe obesity (≥ 40 kg/m 2 )

    Article Snippet: Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland).

    Techniques: Variant Assay

    BBS9 Thr549Ile carrier pedigrees. The black symbols in the pedigrees are heterozygous carriers at Thr549Ile; the white symbols are homozygous at Thr549Thr; the gray symbols are genotype unknown. Maximum BMI (kg/m 2 ), age at maximum BMI, and serum leptin (ng/mL) measures are shown for each participant, if known.

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    doi: 10.1002/oby.23115

    Figure Lengend Snippet: BBS9 Thr549Ile carrier pedigrees. The black symbols in the pedigrees are heterozygous carriers at Thr549Ile; the white symbols are homozygous at Thr549Thr; the gray symbols are genotype unknown. Maximum BMI (kg/m 2 ), age at maximum BMI, and serum leptin (ng/mL) measures are shown for each participant, if known.

    Article Snippet: Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland).

    Techniques:

    The effect of the BBS9 variants on STAT3 transcription in HEK293 cells by an in vitro luciferase activity assay. Each bar represents the mean ± SD of luciferase activity, normalized to the leptin‐treated (100 ng/mL) BBS9 WT from five independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    doi: 10.1002/oby.23115

    Figure Lengend Snippet: The effect of the BBS9 variants on STAT3 transcription in HEK293 cells by an in vitro luciferase activity assay. Each bar represents the mean ± SD of luciferase activity, normalized to the leptin‐treated (100 ng/mL) BBS9 WT from five independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Article Snippet: Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland).

    Techniques: In Vitro, Luciferase, Activity Assay

    The effect of the BBS9 variants on STAT3 phosphorylation in HEK293 cells by Western blot analyses. Each bar represents the mean ± SD of signal intensity normalized to the leptin‐treated BBS9 WT; derived from three independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Exome Sequencing of 21 Bardet‐Biedl Syndrome (BBS) Genes to Identify Obesity Variants in 6,851 American Indians

    doi: 10.1002/oby.23115

    Figure Lengend Snippet: The effect of the BBS9 variants on STAT3 phosphorylation in HEK293 cells by Western blot analyses. Each bar represents the mean ± SD of signal intensity normalized to the leptin‐treated BBS9 WT; derived from three independent experiments. Significant changes (%) in activity from the BBS9 WT were determined by a one‐sample t test. WT, wild type.

    Article Snippet: Expression vectors containing full‐length human myc‐DDK‐tagged LEPR and BBS9 were obtained from Origene (Rockville, Maryland).

    Techniques: Phospho-proteomics, Western Blot, Derivative Assay, Activity Assay